12th Bio Botany one marks – Lesson 4 – Online Test | Principles and Processes of Biotechnology

12th Bio Botany one marks – Lesson 4 – Online Test | Principles and Processes of Biotechnology

12th Bio Botany one marks – Lesson 4 – Online Test | Principles and Processes of Biotechnology Click Here Biology Full Online Test Both Bio Botany and Bio zoology.

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• A Glory has Departed – Jawaharlal Nehru

  2022-12-28

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#1. Restriction enzymes are ___________

Not always required in genetic engineering

Not always required in genetic engineering

Essential tools in genetic engineering

Essential tools in genetic engineering

Nucleases that cleave DNA at specific sites

Nucleases that cleave DNA at specific sites

both b and c

both b and c

#2. Plasmids are ___________

circular protein molecules

circular protein molecules

required by bacteria

required by bacteria

tiny bacteria

tiny bacteria

confer resistance to antibiotics

confer resistance to antibiotics

#3. EcoRI cleaves DNA at

AGGGTT

AGGGTT

GTATATC

GTATATC

GAATTC

GAATTC

TATAGC

TATAGC

#4. Genetic engineering is ___________

making artificial genes

making artificial genes

hybridization of DNA of one organism to that of the others

hybridization of DNA of one organism to that of the others

production of alcohol by using micro organisms

production of alcohol by using micro organisms

making artificial limbs, diagnostic instruments such as ECG and EEG, etc.

making artificial limbs, diagnostic instruments such as ECG and EEG, etc.

#5. Consider the following statements: i. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro ii. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid. iii. Restriction enzymes belong to a class of enzymes called nucleases. Choose the correct option regarding the above statements

i and ii

i and ii

i and iii

i and iii

ii and iii

ii and iii

i,ii, and iii

i,ii, and iii

#6. The process of recombinant DNA technology has the following steps i. Amplification of the gene. ii. Insertion of recombinant DNA into the host cells. iii. Cutting of DNA at a specific location using a restriction enzyme. iv. Isolation of genetic material (DNA). Pick out the correct sequence of steps for recombinant DNA technology.

ii, iii, iv, and i

ii, iii, iv, and i

iv, ii, iii, and i

iv, ii, iii, and i

i, ii, iii and iv

i, ii, iii and iv

iv, iii, i, and ii

iv, iii, i, and ii

#7. Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzymes?

5′ CGTTCG 3′ ATCGTA5′

5′ CGTTCG 3′ ATCGTA5′

5′ GATATG 3′ CTACTA5′

5′ GATATG 3′ CTACTA5′

5′ GAATTC 3′ CTTAAG 5′

5′ GAATTC 3′ CTTAAG 5′

5′ CACGTA 3′ CTCAGT 5′

5′ CACGTA 3′ CTCAGT 5′

#8. pBR 322, BR stands for

Plasmid Bacterial Recombination

Plasmid Bacterial Recombination

Plasmid Bacterial Replications

Plasmid Bacterial Replications

Plasmid Boliver and Rodriguez

Plasmid Boliver and Rodriguez

Plasmid Baltimore and Rodriguez

Plasmid Baltimore and Rodriguez

#9. Which of the following one is used as a Biosensors?

Electrophoresis

Electrophoresis

Bioreactors

Bioreactors

Vectors

Vectors

Electroporation

Electroporation

#10. In which techniques Ethidium Bromide is used?

Southern Blotting techniques

Southern Blotting techniques

Western Blotting techniques

Western Blotting techniques

Polymerase Chain Reaction

Polymerase Chain Reaction

Agarose Gel Electrophoresis

Agarose Gel Electrophoresis

#11. Assertion: Agrobacterium tumifaciens is popular in genetic engineering because of this bacterium is associated with the root nodules of all cereals and pulse crops. Reason: A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the cross with which bacterium is associated.

Both assertion and reason are true. But reason is the correct explanation of assertion.

Both assertion and reason are true. But reason is the correct explanation of assertion.

Both assertion and reason are true. But reason is not a correct explanation of assertion.

Both assertion and reason are true. But reason is not a correct explanation of assertion.

Assertion is true, but reason is false

Assertion is true, but reason is false

Assertion is false, but reason is true.

Assertion is false, but reason is true.

Both assertion and reason are false.

Both assertion and reason are false.

#12. Which one of the following is not correct statement?

Ti plasmid causes the bunchy top disease

Ti plasmid causes the bunchy top disease

Multiple cloning site is known as Polylinker

Multiple cloning site is known as Polylinker

Non-viral method of transfection of Nucleic acid in cell (d) Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

Non-viral method of transfection of Nucleic acid in cell (d) Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

#13. An analysis of chromosomal DNA using the southern hybridisation technique does not use

Electrophoresis

Electrophoresis

Blotting

Blotting

Autoradiography

Autoradiography

Polymerase Chain Reaction

Polymerase Chain Reaction

#14. An antibiotic gene in a vector usually helps in the selection of

Competent cells

Competent cells

Transformed cells

Transformed cells

Recombinant cells

Recombinant cells

None of the above

None of the above

#15. Some of the characteristics of Bt cotton are

Long fibre and resistant to aphids

Long fibre and resistant to aphids

Medium yield, long fibre and resistant to beetle pests

Medium yield, long fibre and resistant to beetle pests

high yield and production of toxic protein crystals which kill dipteran pests.

high yield and production of toxic protein crystals which kill dipteran pests.

High yield and resistant to ball worms

High yield and resistant to ball worms

Finish